Mixing insulins in one syringe are not hard to learn; it just takes a little bit of practice. Once you go through the instructions to mix insulin in a syringe given below, you will find it is an effortless task.

Example of Dose

The doctor ordered 10 units Humulin R insulin and 30 units Humulin N insulin subcutaneously, 30 minutes before breakfast.

Since the doctor ordered two different insulin preparations to be mixed in the same syringe and your stock is not premixed. They need to be mixed manually in one syringe. Mixing insulin requires extra steps that must be completed in a specific order.

10 units of clear [ R(Regular) insulin]
+   30 units of cloudy  [N(NPH) insulin]
=   40 total units.  [Total mixe dose]

To administer the dose use 50 unit of Lo-Dose syringe

How would you prepare to administer this injection?


  1. Gather all of the equipment you will need
  2. Wash your hands
  3. Check the expiration date on the vials of insulin. If they are not expired, pick up the cloudy bottle of insulin and roll it between your hands until it is mixed, until there is no powder on the bottom of the bottle. Do not shake the insulin bottle because this can cause air bubbles.
  4. Take the bottle of cotton ball dipped in alcohol and wipe the top of both insulin vials
  5. Pick the Humulin N (slower acting insulin) first and inject 30 units of air into the vial

    Mixing two types of insulin in one syringe
    Mixing two types of insulin in one syringe

  6. After putting air in the Humulin N(slower acting insulin)  do not draw up the insulin yet, just pull the needle out and insert 10 units of air into the Humulin R( faster-acting insulin) vial.
  7. Then, after you inject the air into Humulin R (faster-acting insulin) turn the vial upside down to draw 10 units of Humulin R( faster-acting insulin) into the syringe.
  8. Once you have drawn up yours 10 units, Insert the needle into the vial of Humulin N (slower-acting insulin) and draw the slower-acting insulin into the syringe(in this case,30 units for a total 40 mixed-dose unit into the syringe)

This is all you need to do when mixing insulin in one syringe. Just follow the correct order. Specifically, air must be injected into the vial of the slower-acting insulin first, and faster-acting insulin must be drawn into the syringe first.


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